DETECTION OF A NUCLEIC ACID SEQUENCE OR A CHANGE THEREIN

申请公布号：: CA2044591(A1)
申请号：: CA19902044591
申请日期：: 1990.02.13
申请公布日期：: 1990.08.14
申请人：: GENECO PTY LTD.
发明人：: DALE, JAMES L.;TIMMS, PETER;WALSH, TERENCE P.
分类号：: G01N33/53;C12M1/00;C12Q1/68;C12Q1/70;G01N1/28;(IPC1-7):C12Q1/68
主分类号：: G01N33/53
摘要：: A method for detecting a specific polynucleotide sequence in a material is disclosed. The method includes exposing said material to an oligonucleotide primer having a sequence complementary to par t of said specific polynucleotide sequence wherein said primer binds to pan of said polynucleotide sequence when present in sai d material. Primer bound to the polynucleotide se- quence is extended wherein any extended primer includes a detectable element and/or a separation element. Any extended pri- mer is then separated into a fraction wherein said fraction does not have detectable element not included in said extended primer. One then determines whether any extended primer is present in said fraction by assaying said fraction for said extended primer wherein the presence of said extended primer is indicative of the presence o f the specific polynucleotide sequence in said material and the absence of said extended primer in said fraction is indicative of th e absence of the specific polynucleotide sequence in said material.