System for screening fatty acid transport inhibitors, methods of use and modulators identified thereby
- 申请公布号:
- US7070944(B2)
- 申请号:
- US20020099350
- 申请日期:
- 2002.03.14
- 申请公布日期:
- 2006.07.04
- 申请人:
- CLF MEDICAL TECHNOLOGY ACCELERATION PROGRAM, INC.
- 发明人:
- BLACK PAUL;DIRUSSO CONCETTA
- 分类号:
- G01N33/53;C12N1/20;C12N5/22;C12Q1/25;G01N33/92
- 主分类号:
- G01N33/53
- 摘要:
- Methods for identifying modulators of fatty acid transport and/or uptake, comprising contacting, under conditions favorable for fatty acid uptake, a putative modulator, e.g., an inhibitor with a system comprising genetic material encoding a fatty acid transport mediator ("TTM"), particularly FAA1, FAA2, FAA3, FAA4, FAT1, fadL, fadD, FATP, CD36 and FABP, or orthologs, homologs, isoforms, variants, analogs, derivatives or fragments thereof, and combinations thereof, or fatty acid transport mediator proteins ("TTMps"), e.g., Faa1p, Faa2p, Faa3p, Faa4p, Fat1p, FadL, fatty acyl CoA synthetase, FATP, CD36 and FABP, or orthologs, homologs, isoforms, variants, analogs, derivatives or fragments thereof, and combinations thereof, and determining the effect of the putative modulator. The test system may be a cell such as Saccharomyces cerevisiae, E. coli, H. sapiens, etc. or an in vitro system.
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