SYSTEM AND METHOD FOR MOVEMENT AND POSITIONING OF REACTION MIXTURE DURING NUCLEIC ACID AMPLIFICATION
- 申请公布号：
- US2010129872(A1)
- 申请号：
- US20090624334
- 申请日期：
- 2009.11.23
- 申请公布日期：
- 2010.05.27
- 申请人：
- GHC TECHNOLOGIES, INC.
- 发明人：
- PAIK PHILIP Y.;MEYER MICHAEL R.
- 分类号：
- C12P19/34;C12M1/00
- 主分类号：
- C12P19/34
- 摘要：
- A system for accurately positioning a reaction mixture during amplification of a nucleic acid includes a reaction vessel that contains the reaction mixture, a first heat exchanger, a second heat exchanger and a pump assembly. The reaction vessel can include .a first zone and a second zone. The first heat exchanger is positioned near the first zone, and the second heat exchanger positioned near the second zone. The first heat exchanger adjusts the temperature of the reaction mixture so the reaction mixture is at a first temperature when the reaction mixture is in the first zone. The second heat exchanger adjusts the temperature of the reaction mixture so the reaction mixture is at a second temperature. The pump assembly adjusts the pressure within the reaction vessel to selectively position the reaction mixture relative to the first zone and the second zone during amplification. The system can include a sensor that monitors the position of the reaction mixture within the reaction vessel. The system can also include a second pump that cooperates with the pump assembly to adjust the position of the reaction mixture within the reaction vessel.
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