TARGET PREPARATION FOR PARALLEL SEQUENCING OF COMPLEX GENOMES
- 申请公布号：
- US2010204050(A1)
- 申请号：
- US20100689269
- 申请日期：
- 2010.01.19
- 申请公布日期：
- 2010.08.12
- 申请人：
- DONNER HORST;BUCHBERGER BERND
- 发明人：
- DONNER HORST;BUCHBERGER BERND
- 分类号：
- C40B20/02;C12Q1/68;C40B40/06
- 主分类号：
- C40B20/02
- 摘要：
- The present invention provides a method for the isolation and analysis of a target nucleic acid, the target nucleic acid being present in a sample of genomic DNA, comprising the steps of a) fragmentation of the genomic DNA, b) hybridization of the genomic DNA on a nucleic acid solid support, the solid support comprising a plurality of oligonucleotide probes, the probes being characterized in that each probe is at least partially complementary to the sequence of the target nucleic acid or its complement, under hybridization conditions, characterized in that the plurality of probes hybridizes to fragments of the target nucleic acid but does not hybridize to other nucleic acids which are present in the sample, c) stripping off the target molecules hybridized to the nucleic acid array, d) overlap extension synthesis in order to generate double stranded overlap extension synthesis product, e) fragment polishing, and f) adaptor ligation.
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