Simultaneous detection of metabolic enzyme activity and metabolite levels
- 申请公布号：
- US8318417(B2)
- 申请号：
- US20090349669
- 申请日期：
- 2009.01.07
- 申请公布日期：
- 2012.11.27
- 申请人：
- CERDA BLAS;PERKINELMER HEALTH SCIENCES, INC.
- 发明人：
- CERDA BLAS
- 分类号：
- C12Q1/00;C12Q1/34;C12Q1/68;G01N33/00;G01N33/53
- 主分类号：
- C12Q1/00
- 摘要：
- Provided are methods for detecting a metabolic disorder in an individual using mass spectrometry. One method involves (a) contacting a sample containing (i) a metabolically indicative enzyme and (ii) a metabolic analyte, with a substrate for the enzyme to produce a reaction admixture, under conditions wherein the enzyme is capable of acting on a corresponding substrate to generate a product, and wherein a protease inhibitor is present; (b) contacting the reaction admixture with a reagent that inhibits the ability of the enzyme to act on a corresponding substrate, wherein the metabolic analyte and the product are soluble in the reagent; to produce a test sample and (c) determining the presence or amount of the metabolic analyte and the product contained in the test sample using mass spectrometry, wherein a determined presence or amount of the metabolic analyte and the product correlates with presence or absence of the metabolic disorder.
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