Method for isolating a part of a layer of a biological material
- 申请公布号：
- US8845623(B2)
- 申请号：
- US200711671702
- 申请日期：
- 2007.02.06
- 申请公布日期：
- 2014.09.30
- 申请人：
- Molecular Machines & Industries AG
- 发明人：
- Leclerc Norbert
- 分类号：
- A61B18/18;G01N1/28
- 主分类号：
- A61B18/18
- 代理机构：
- Henry M. Feiereisen LLP
- 代理人：
- Henry M. Feiereisen LLP ;Day Ursula B.
- 地址：
- Glattbrugg CH
- 摘要：
- A film with a tissue section is placed with the tissue section downward on a microscope slide and the microscope slide positioned in the object plane of an inverse microscope; where an adhesive tape is arranged, with a bonding agent downward, above the film and therefore above the tissue section; wherein the next step, tissue to be isolated is excised by a focused laser beam, which also divides the film; whereupon removal of the adhesive tape from the microscope, the excised tissue pieces adhere to the adhesive tape, and the remnant of the film and of the tissue section remains adhering to the microscope slide.
- 主权项：
- 1. A method for isolating a part of a layer of biological material, comprising the steps of:
a) applying the layer of biological material to one side of a stabilization layer; b) positioning the stabilization layer with the side facing away from the layer of biological material suspended above a catching device in the form of a slide, said catching device including a flat retaining part; wherein spacers are positioned between the stabilization layer and the catching device inserted between the stabilization layer and the slide; c) focusing a laser beam and describing a closed curve around the part of the stabilization layer with the biological material to be isolated, and eroding the biological material as well as the stabilization layer on the curve thus separating the part to be isolated from a remnant of the stabilization layer and biological material; and d) catching the part to be isolated on the catching device.
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