Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor
- 申请公布号：
- US5236824(A)
- 申请号：
- US19910812132
- 申请日期：
- 1991.12.18
- 申请公布日期：
- 1993.08.17
- 申请人：
- NIPPON TELEGRAPH AND TELEPHONE CORPORATION
- 发明人：
- FUJIWARA, KOICHI;MIZUTANI, HIROKO;MIZUTANI, DECEASED, HIROMICHI;SHIBATA, SHUICHI;ARISHIMA, KOICHI;HOSHINO, MITSUTOSHI;HOSHINO, YASUSHI
- 分类号：
- G01N33/543;G01N33/58;G01N35/00
- 主分类号：
- G01N33/543
- 摘要：
- An in-situ laser magnetic immunoassay ("LMIA") method which eliminates the step of B/F separation generally required in the labeling method of immunoassays. The laser magnetic immunoassay permits a quantitative determination of a target immunological substance, for example, an antigen, an antibody, lymphocytes, viruses, tumorous cells and infections cells, in an analyte solution containing both bound and free species. A transitory increase in the magnetophoretic scattering of laser beam is observed when the analyte solution contains magnetic-labeled, bound target analyte, while no such increase is observed in a control test solution, containing only the relevant reagents. A magnetophoretic LMIA apparatus is provided which includes a magnetic gradient generating device which forms an integral part of the in-situ LMIA.
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