TRANSFER DEVICE FOR TWO - DIMENSIONAL ELECTROPHORESIS
- 申请公布号：
- WO2007106370(A9)
- 申请号：
- WO2007US05966
- 申请日期：
- 2007.03.08
- 申请公布日期：
- 2007.12.13
- 申请人：
- PROTEIN FOREST, INC.;HARALAMPU, STEPHEN;SKEA, WILLIAM, M.;CHLENOV, MARK;THOMPSON, EDWARD
- 发明人：
- HARALAMPU, STEPHEN;SKEA, WILLIAM, M.;CHLENOV, MARK;THOMPSON, EDWARD
- 分类号：
- G01N27/447;C07K1/26
- 主分类号：
- G01N27/447
- 摘要：
- Apparatus and method for transfer of analytes from a first dimension isoelectric focusing gel to a second dimension, such as an electrophoretic analytical gel. In a preferred embodiment, the device (10) facilitates the transfer of protein analytes from a digital proteome chip (12) to a second dimension electrophoretic analytical gel (18). The main features of the preferred embodiment are a recessed location (14) on a collar (16a, 16b), a mechanical clamp device (22) and stops (20a, 20b) that locate the first dimension gel at a fixed and known position in space, and a low density medium that assures uniform electrical contact between the first and second dimension analyses.The advantage of an apparatus that places the first dimension in a known position is that the final data analysis is greatly facilitated by precisely knowing the locations of the first dimension pH features. The medium that assures electrical contact also has the advantage of simplifying the final data analysis by assuring a uniform frame of reference on how the second dimension analysis was run.
Copyright Notice © 2009-2024 传众 版权所有